Effects of a centipede venom fraction on insect nervous system, a native Xenopus oocyte receptor and on an expressed Drosophila muscarinic receptor
Identifieur interne : 000316 ( France/Analysis ); précédent : 000315; suivant : 000317Effects of a centipede venom fraction on insect nervous system, a native Xenopus oocyte receptor and on an expressed Drosophila muscarinic receptor
Auteurs : Maria Stankiewicz [Pologne] ; Alain Hamon [France] ; Rym Benkhalifa [France, Tunisie] ; Wojciech Kadziela [Pologne] ; Bernard Hue [France] ; Sylvia Lucas [Brésil] ; Dietrich Mebs [Allemagne] ; Marcel Pelhate [France]Source :
- Toxicon [ 0041-0101 ] ; 1999.
Abstract
Centipede venoms are complex protein mixtures; very few is known about their pharmacological actions. Application of a Scolopendra sp. venom fraction (SC1) on the cockroach giant axon induced an increase in the leak current correlated with a decrease in the membrane resistance, suggesting the presence in SC1 of components opening non-specific pores in the axonal membrane. On a cockroach central cholinergic synapse, microinjection of SC1 induced a small transient depolarization of the postsynaptic membrane, followed by a slow stable depolarization and a drastic decrease in the evoked subthreshold excitatory postsynaptic potential amplitude. A pretreatment of the ganglion with atropine or scopolamine reduced the amplitude of the SC1-induced depolarizing wave, suggesting a possible cholinergic muscarinic target. On control Xenopus oocytes, SC1 induced an inward oscillatory Ca2+-dependent Cl− current mediated through the activation of native lysophosphatidic acid receptors (LPAr). Indeed, pretreatment of oocytes with 1 μM N-palmitoyl-tyrosine phosphoric acid, a selective competitive antagonist of LPAr, decreased responses to SC1 by 70%. Application of SC1 to oocytes expressing a cloned Drosophila muscarinic receptor (Dm1) induced a biphasic response comprising: (1) a large fast Cl− current that was abolished by pretreatment with atropine and scopolamine and (2) a slow and small oscillating Cl− current corresponding to the response observed in control oocytes. These observations confirm the presence of muscarinic agonists in SC1 and reveal their direct action on an insect muscarinic receptor subtype homologous to mammalian M1–M3 receptors.
Url:
DOI: 10.1016/S0041-0101(99)00089-6
Affiliations:
- Allemagne, Brésil, France, Pologne, Tunisie
- District de Darmstadt, Hesse (Land), Pays de la Loire, État de São Paulo
- Angers, Francfort-sur-le-Main, São Paulo
- Université d'Angers
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Application of a Scolopendra sp. venom fraction (SC1) on the cockroach giant axon induced an increase in the leak current correlated with a decrease in the membrane resistance, suggesting the presence in SC1 of components opening non-specific pores in the axonal membrane. On a cockroach central cholinergic synapse, microinjection of SC1 induced a small transient depolarization of the postsynaptic membrane, followed by a slow stable depolarization and a drastic decrease in the evoked subthreshold excitatory postsynaptic potential amplitude. A pretreatment of the ganglion with atropine or scopolamine reduced the amplitude of the SC1-induced depolarizing wave, suggesting a possible cholinergic muscarinic target. On control Xenopus oocytes, SC1 induced an inward oscillatory Ca2+-dependent Cl− current mediated through the activation of native lysophosphatidic acid receptors (LPAr). Indeed, pretreatment of oocytes with 1 μM N-palmitoyl-tyrosine phosphoric acid, a selective competitive antagonist of LPAr, decreased responses to SC1 by 70%. Application of SC1 to oocytes expressing a cloned Drosophila muscarinic receptor (Dm1) induced a biphasic response comprising: (1) a large fast Cl− current that was abolished by pretreatment with atropine and scopolamine and (2) a slow and small oscillating Cl− current corresponding to the response observed in control oocytes. These observations confirm the presence of muscarinic agonists in SC1 and reveal their direct action on an insect muscarinic receptor subtype homologous to mammalian M1–M3 receptors.</div></front></TEI><affiliations><list><country><li>Allemagne</li><li>Brésil</li><li>France</li><li>Pologne</li><li>Tunisie</li></country><region><li>District de Darmstadt</li><li>Hesse (Land)</li><li>Pays de la Loire</li><li>État de São Paulo</li></region><settlement><li>Angers</li><li>Francfort-sur-le-Main</li><li>São Paulo</li></settlement><orgName><li>Université d'Angers</li></orgName></list><tree><country name="Pologne"><noRegion><name sortKey="Stankiewicz, Maria" sort="Stankiewicz, Maria" uniqKey="Stankiewicz M" first="Maria" last="Stankiewicz">Maria Stankiewicz</name></noRegion><name sortKey="Kadziela, Wojciech" sort="Kadziela, Wojciech" uniqKey="Kadziela W" first="Wojciech" last="Kadziela">Wojciech Kadziela</name></country><country name="France"><region name="Pays de la Loire"><name sortKey="Hamon, Alain" sort="Hamon, Alain" uniqKey="Hamon A" first="Alain" last="Hamon">Alain Hamon</name></region><name sortKey="Benkhalifa, Rym" sort="Benkhalifa, Rym" uniqKey="Benkhalifa R" first="Rym" last="Benkhalifa">Rym Benkhalifa</name><name sortKey="Hamon, Alain" sort="Hamon, Alain" uniqKey="Hamon A" first="Alain" last="Hamon">Alain Hamon</name><name sortKey="Hue, Bernard" sort="Hue, Bernard" uniqKey="Hue B" first="Bernard" last="Hue">Bernard Hue</name><name sortKey="Pelhate, Marcel" sort="Pelhate, Marcel" uniqKey="Pelhate M" first="Marcel" last="Pelhate">Marcel Pelhate</name></country><country name="Tunisie"><noRegion><name sortKey="Benkhalifa, Rym" sort="Benkhalifa, Rym" uniqKey="Benkhalifa R" first="Rym" last="Benkhalifa">Rym Benkhalifa</name></noRegion></country><country name="Brésil"><region name="État de São Paulo"><name sortKey="Lucas, Sylvia" sort="Lucas, Sylvia" uniqKey="Lucas S" first="Sylvia" last="Lucas">Sylvia Lucas</name></region></country><country name="Allemagne"><region name="Hesse (Land)"><name sortKey="Mebs, Dietrich" sort="Mebs, Dietrich" uniqKey="Mebs D" first="Dietrich" last="Mebs">Dietrich Mebs</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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